You can connect to BaseSpace at basespace.illumina.com. Log in using your MyIllumina account login. For
more information on BaseSpace, see the
BaseSpace User Guide (document # 15044182)
and the
BaseSpace support pages on the Illumina website.
For more information on BaseSpace Onsite, see the
BaseSpace Onsite System Guide (document #
15049148)
and the BaseSpace OnSite support pages on the Illumina website.
MiSeq Reporter Software
MiSeq Reporter is a Windows Service application that processes base calls generated by RTA software.
MiSeq Reporter begins secondary analysis immediately after the completion of analysis of the sequencing run
by the RTA software.
MiSeq Reporter runs on the instrument computer. However, the software interface must be viewed through a
web browser on another computer that is connected to the same network as the MiSeq Reporter.
When secondary analysis is complete, a file named CompletedJobInfo.xml is written to the run folder. For
more information, see the
MiSeq Reporter Software Guide (document #15042295)
.
Sequencing During Analysis
The MiSeq system computing resources are dedicated to either sequencing or analysis.
With MiSeq Reporter, if a new sequencing run is started on the MiSeq before secondary analysis of an earlier
run is complete, MiSeq Reporter analysis is automatically stopped.
To restart MiSeq Reporter, use the Requeue feature after the new sequencing run is complete.
After the new run completes sequencing, secondary analysis of the earlier run automatically starts again from
the beginning.
Sequencing Analysis Viewer
You can monitor your run in greater detail without interfering with the run using the Illumina Sequencing
Analysis Viewer (SAV). Your MiSeq must be networked to view primary analysis results with SAV.
SAV allows you to review metrics during a run as metrics are generated, and later after a run has completed.
Install SAV onto a computer independent of the MiSeq with access to the same network connected to the
instrument. After launching the software, browse to the output folder for your run.
After template generation, SAV provides metrics generated by RTA and organizes the metrics into plots,
graphs, and tables.
NOTE
SAV is universal to Illumina sequencing systems, most of which use an 8-lane flow cell. Some views include
drop-down lists showing lanes 1–8. Because the MiSeq flow cell has a single lane, select All or Lane 1. For
more information, see the
Sequencing Analysis Viewer User Guide (document # 15020619)
.
Required Disk Space
The integrated instrument computer has approximately 550GB of storage capacity.
Before starting a run, the software checks available disk space. If there is not enough disk space for the run,
a message indicating how much disk space is required appears.
If prompted to make disk space available, move or delete older run folders as appropriate. For more
information, see
Manage Files
on page 38. After clearing adequate disk space, select Restart Check.
Document # 1000000061014 v00
For Research Use Only. Not
for use in diagnostic procedures.
8
MiSeq System Guide